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truA [2019-07-19 11:48:42]
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truA [2019-07-19 11:48:42]

pseudouridylate synthase I, universally conserved protein
Locus
BSU_01480
Isoelectric point
6.65
Molecular weight
27.90 kDa
Protein length
247 aa Sequence Blast
Gene length
744 bp Sequence Blast
Function
tRNA modification
Product
pseudouridylate synthase I
Essential
no
E.C.
5.4.99.12
Synonyms
ybaH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation
  • Groups of genes → Universally conserved proteins

    • Gene

    Coordinates
    152,937 153,680

    The protein

    Catalyzed reaction/ biological activity

  • tRNA uridine = tRNA pseudouridine (according to Swiss-Prot)

    • Protein family

  • tRNA pseudouridine synthase TruA family (single member, according to UniProt)

    • Structure

  • 1VS3 (from Thermus thermophilus, 42% identity) PubMed

    • Additional information

  • universally conserved protein

    • Expression and Regulation

    Operons

    Genes
    truA-rplM-rpsI
    Description
    PubMed

    Regulatory mechanism

  • stringent response: negative regulation, PubMed, in stringent response

    • Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed
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    Biological materials

    Mutant

  • BKE01480 (truA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACAGCACCTCTAAGCCC, downstream forward: _UP4_TAAACCAGGTGTATAATATT
  • BKK01480 (truA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACAGCACCTCTAAGCCC, downstream forward: _UP4_TAAACCAGGTGTATAATATT

    • References

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